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pegfp c1 mammalian expression vectors  (TaKaRa)


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    TaKaRa pegfp c1 mammalian expression vectors
    Pegfp C1 Mammalian Expression Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+c1+mammalian+expression+vector/pm37723223-342-11-15
    Average 86 stars, based on 1 article reviews
    pegfp c1 mammalian expression vectors - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Lamin A Rod Domain Mutants Target Heterochromatin Protein 1α and β for Proteasomal Degradation by Activation of F-Box Protein, FBXW10
    Article Snippet: The 3-kb FBXW10 gene was initially amplified by PCR as three separate fragments, using PhusionTM high fidelity DNA polymerase (Finnzymes) as per the manufacturer's instructions and with the appropriate primers listed below, followed by cloning into pMOS vector. .. Then the middle and 3′ fragments were reamplified by overlap PCR to give a single fragment which was ligated to the 5′ fragment after appropriate enzyme digestion and cloned into XhoI, BamHI-digested pEGFP-C1 mammalian expression vector (Clontech) and recloned into an mRFP mammalian expression vector (Clontech). ..

    Article Title: Transgenic Mice Overexpressing PG1 Display Corneal Opacity and Severe Inflammation in the Eye
    Article Snippet: .. Coding sequences of pre-pro and mature PG1 were amplified from pig PBMC cDNA via PCR using the primer sets pPG-F and –R, mPG-F (5′-ACT-CAG-ATC-TGG-TGG-CGG-TT-3′) and pPG-R. Amplicons were then subcloned into the multiple cloning site of the pEGFP-C1 mammalian expression vector (Clontech Laboratories, Inc., Mountain View, CA, USA). .. NIH3T3 (ATCC, Manassas, VA, USA) and 661W cells, which were shown to be derived from the cone photoreceptor cell lineage [ ], were transfected with the plasmid construct using PolyMagTM transfection reagent (Chemicell, Berlin, Germany) following the manufacturer protocol.

    Article Title: Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells
    Article Snippet: .. Affimer sequences were subcloned into the pEGFP-C1 mammalian expression vector (Clontech) by a PCR based approach. .. Sequences lacking the His-tag and cysteine residue were amplified and the resulting cDNA inserted into the EcoRI and BamHI restriction sites, downstream of and in frame with the eGFP coding sequence. mEGFP-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54610, derived from a 17 amino acid residue actin-binding peptide fused to the C-terminus of eGFP).

    Clone Assay:

    Article Title: Lamin A Rod Domain Mutants Target Heterochromatin Protein 1α and β for Proteasomal Degradation by Activation of F-Box Protein, FBXW10
    Article Snippet: The 3-kb FBXW10 gene was initially amplified by PCR as three separate fragments, using PhusionTM high fidelity DNA polymerase (Finnzymes) as per the manufacturer's instructions and with the appropriate primers listed below, followed by cloning into pMOS vector. .. Then the middle and 3′ fragments were reamplified by overlap PCR to give a single fragment which was ligated to the 5′ fragment after appropriate enzyme digestion and cloned into XhoI, BamHI-digested pEGFP-C1 mammalian expression vector (Clontech) and recloned into an mRFP mammalian expression vector (Clontech). ..

    Article Title: Takinib, a selective TAK1 inhibitor, broadens the therapeutic efficacy of TNF α inhibition for cancer and autoimmune disease
    Article Snippet: .. The P. falciparum PK9 gene (PlasmoDB accession # PF3D7_1315100) was cloned into the pEGFP-C1 mammalian expression vector (Clontech) in frame with a N-terminal GFP tag. .. Parental HEK293 cells were transfected with the resulting PfPK9-pEGFP-C1 vector to generate HEK293-GFP-PfPK9 expressing cells using X-tremeGENE HP transfection reagent (Roche) per manufacturer instructions.

    Article Title: A Disease-associated Mutant of NLRC4 Shows Enhanced Interaction with SUG1 Leading to Constitutive FADD-dependent Caspase-8 Activation and Cell Death
    Article Snippet: Cy3 (Indocarbocyanine) and Alexa 633- conjugated mouse or rabbit secondary antibodies were purchased from Millipore and Thermo Fisher, respectively. .. Expression Vectors—Full-length NLRC4 cDNA, various deletion constructs, and NLRC4-Ub2 cloned in pEGFP-C1 mammalian expression vector (Clontech) are described (15). ..

    Article Title: The dimerization domain of SOX9 is required for transcription activation of a chondrocyte-specific chromatin DNA template
    Article Snippet: .. These Flag-tagged SOX9 were also linked to 3 × HA tag at the C-terminus and cloned into EcoRI and BamHI sites of modified pEGFP-C1 mammalian expression vector (Clontech) that was deleted of the EGFP sequence. ..

    Expressing:

    Article Title: Lamin A Rod Domain Mutants Target Heterochromatin Protein 1α and β for Proteasomal Degradation by Activation of F-Box Protein, FBXW10
    Article Snippet: The 3-kb FBXW10 gene was initially amplified by PCR as three separate fragments, using PhusionTM high fidelity DNA polymerase (Finnzymes) as per the manufacturer's instructions and with the appropriate primers listed below, followed by cloning into pMOS vector. .. Then the middle and 3′ fragments were reamplified by overlap PCR to give a single fragment which was ligated to the 5′ fragment after appropriate enzyme digestion and cloned into XhoI, BamHI-digested pEGFP-C1 mammalian expression vector (Clontech) and recloned into an mRFP mammalian expression vector (Clontech). ..

    Article Title: Takinib, a selective TAK1 inhibitor, broadens the therapeutic efficacy of TNF α inhibition for cancer and autoimmune disease
    Article Snippet: .. The P. falciparum PK9 gene (PlasmoDB accession # PF3D7_1315100) was cloned into the pEGFP-C1 mammalian expression vector (Clontech) in frame with a N-terminal GFP tag. .. Parental HEK293 cells were transfected with the resulting PfPK9-pEGFP-C1 vector to generate HEK293-GFP-PfPK9 expressing cells using X-tremeGENE HP transfection reagent (Roche) per manufacturer instructions.

    Article Title: Tocotrienols prevent hydrogen peroxide-induced axon and dendrite degeneration in cerebellar granule cells.
    Article Snippet: .. A pEGFP-C1 mammalian expression vector (Clontech Takara Bio Inc., Tokyo, Japan) was added to 25 μ L of opti-MEM I Life Technologies reduced serum medium. ..

    Article Title: A Disease-associated Mutant of NLRC4 Shows Enhanced Interaction with SUG1 Leading to Constitutive FADD-dependent Caspase-8 Activation and Cell Death
    Article Snippet: Cy3 (Indocarbocyanine) and Alexa 633- conjugated mouse or rabbit secondary antibodies were purchased from Millipore and Thermo Fisher, respectively. .. Expression Vectors—Full-length NLRC4 cDNA, various deletion constructs, and NLRC4-Ub2 cloned in pEGFP-C1 mammalian expression vector (Clontech) are described (15). ..

    Article Title: Sciatic nerve regeneration by microporous nerve conduits seeded with glial cell line-derived neurotrophic factor or brain-derived neurotrophic factor gene transfected neural stem cells.
    Article Snippet: Neurotrophic factors such as the glial cell linederived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF) promote nerve cell survival and regeneration, but their efficacy in repairing a longer gap defect of rat sciatic nerve (15 mm) has not been established.. In this study, two recombinant mammalian vectors containing either rat GDNF gene or BDNF gene were constructed and each was transfected into neural stem cells (NSCs).. It was found that the transfection of GDNF or BDNF gene into NSCs led to significantly enhanced expression of GDNF or BDNF mRNA.

    Article Title: Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells
    Article Snippet: .. Affimer sequences were subcloned into the pEGFP-C1 mammalian expression vector (Clontech) by a PCR based approach. .. Sequences lacking the His-tag and cysteine residue were amplified and the resulting cDNA inserted into the EcoRI and BamHI restriction sites, downstream of and in frame with the eGFP coding sequence. mEGFP-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54610, derived from a 17 amino acid residue actin-binding peptide fused to the C-terminus of eGFP).

    Plasmid Preparation:

    Article Title: Lamin A Rod Domain Mutants Target Heterochromatin Protein 1α and β for Proteasomal Degradation by Activation of F-Box Protein, FBXW10
    Article Snippet: The 3-kb FBXW10 gene was initially amplified by PCR as three separate fragments, using PhusionTM high fidelity DNA polymerase (Finnzymes) as per the manufacturer's instructions and with the appropriate primers listed below, followed by cloning into pMOS vector. .. Then the middle and 3′ fragments were reamplified by overlap PCR to give a single fragment which was ligated to the 5′ fragment after appropriate enzyme digestion and cloned into XhoI, BamHI-digested pEGFP-C1 mammalian expression vector (Clontech) and recloned into an mRFP mammalian expression vector (Clontech). ..

    Article Title: Takinib, a selective TAK1 inhibitor, broadens the therapeutic efficacy of TNF α inhibition for cancer and autoimmune disease
    Article Snippet: .. The P. falciparum PK9 gene (PlasmoDB accession # PF3D7_1315100) was cloned into the pEGFP-C1 mammalian expression vector (Clontech) in frame with a N-terminal GFP tag. .. Parental HEK293 cells were transfected with the resulting PfPK9-pEGFP-C1 vector to generate HEK293-GFP-PfPK9 expressing cells using X-tremeGENE HP transfection reagent (Roche) per manufacturer instructions.

    Article Title: A Disease-associated Mutant of NLRC4 Shows Enhanced Interaction with SUG1 Leading to Constitutive FADD-dependent Caspase-8 Activation and Cell Death
    Article Snippet: Cy3 (Indocarbocyanine) and Alexa 633- conjugated mouse or rabbit secondary antibodies were purchased from Millipore and Thermo Fisher, respectively. .. Expression Vectors—Full-length NLRC4 cDNA, various deletion constructs, and NLRC4-Ub2 cloned in pEGFP-C1 mammalian expression vector (Clontech) are described (15). ..

    Article Title: The dimerization domain of SOX9 is required for transcription activation of a chondrocyte-specific chromatin DNA template
    Article Snippet: .. These Flag-tagged SOX9 were also linked to 3 × HA tag at the C-terminus and cloned into EcoRI and BamHI sites of modified pEGFP-C1 mammalian expression vector (Clontech) that was deleted of the EGFP sequence. ..

    Article Title: Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells
    Article Snippet: .. Affimer sequences were subcloned into the pEGFP-C1 mammalian expression vector (Clontech) by a PCR based approach. .. Sequences lacking the His-tag and cysteine residue were amplified and the resulting cDNA inserted into the EcoRI and BamHI restriction sites, downstream of and in frame with the eGFP coding sequence. mEGFP-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54610, derived from a 17 amino acid residue actin-binding peptide fused to the C-terminus of eGFP).

    Construct:

    Article Title: A Disease-associated Mutant of NLRC4 Shows Enhanced Interaction with SUG1 Leading to Constitutive FADD-dependent Caspase-8 Activation and Cell Death
    Article Snippet: Cy3 (Indocarbocyanine) and Alexa 633- conjugated mouse or rabbit secondary antibodies were purchased from Millipore and Thermo Fisher, respectively. .. Expression Vectors—Full-length NLRC4 cDNA, various deletion constructs, and NLRC4-Ub2 cloned in pEGFP-C1 mammalian expression vector (Clontech) are described (15). ..

    Amplification:

    Article Title: Transgenic Mice Overexpressing PG1 Display Corneal Opacity and Severe Inflammation in the Eye
    Article Snippet: .. Coding sequences of pre-pro and mature PG1 were amplified from pig PBMC cDNA via PCR using the primer sets pPG-F and –R, mPG-F (5′-ACT-CAG-ATC-TGG-TGG-CGG-TT-3′) and pPG-R. Amplicons were then subcloned into the multiple cloning site of the pEGFP-C1 mammalian expression vector (Clontech Laboratories, Inc., Mountain View, CA, USA). .. NIH3T3 (ATCC, Manassas, VA, USA) and 661W cells, which were shown to be derived from the cone photoreceptor cell lineage [ ], were transfected with the plasmid construct using PolyMagTM transfection reagent (Chemicell, Berlin, Germany) following the manufacturer protocol.

    Cloning:

    Article Title: Transgenic Mice Overexpressing PG1 Display Corneal Opacity and Severe Inflammation in the Eye
    Article Snippet: .. Coding sequences of pre-pro and mature PG1 were amplified from pig PBMC cDNA via PCR using the primer sets pPG-F and –R, mPG-F (5′-ACT-CAG-ATC-TGG-TGG-CGG-TT-3′) and pPG-R. Amplicons were then subcloned into the multiple cloning site of the pEGFP-C1 mammalian expression vector (Clontech Laboratories, Inc., Mountain View, CA, USA). .. NIH3T3 (ATCC, Manassas, VA, USA) and 661W cells, which were shown to be derived from the cone photoreceptor cell lineage [ ], were transfected with the plasmid construct using PolyMagTM transfection reagent (Chemicell, Berlin, Germany) following the manufacturer protocol.

    Modification:

    Article Title: The dimerization domain of SOX9 is required for transcription activation of a chondrocyte-specific chromatin DNA template
    Article Snippet: .. These Flag-tagged SOX9 were also linked to 3 × HA tag at the C-terminus and cloned into EcoRI and BamHI sites of modified pEGFP-C1 mammalian expression vector (Clontech) that was deleted of the EGFP sequence. ..

    Sequencing:

    Article Title: The dimerization domain of SOX9 is required for transcription activation of a chondrocyte-specific chromatin DNA template
    Article Snippet: .. These Flag-tagged SOX9 were also linked to 3 × HA tag at the C-terminus and cloned into EcoRI and BamHI sites of modified pEGFP-C1 mammalian expression vector (Clontech) that was deleted of the EGFP sequence. ..



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